The goal of the Neuron Tracing and Active Learning Environment (NeuroTRALE) project is to provide new insights into brain cellular organization and underlying neuron function, while assessing individual variabilities and changes across lifespans. Our web-based tool helps to manage and visualize large brain imagery datasets in 3D with editable annotations overlaid. The interactive interface is designed to facilitate efficient training and evaluation of algorithms for segmenting and tracing neuron structures in volumetric microscopy images.